Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
P253F/G254A/R258M/L443F | the mutant shows the same thermostability as the wild type enzyme while it displays an extended substrate spectrum | Thermobifida fusca |
W501A | the mutant shows reduced activity compared to the wild type enzyme | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Octanone | substrate inhibition | Thermobifida fusca | |
phenylacetone | substrate inhibition | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
0.08 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
0.25 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
0.8 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
2 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
4 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1000 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1200 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the initial activity of wild type enzyme is not affected by 10% (v/v) 1,4-dioxane | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-methyl-2-pentylcyclopentanone + NADPH + H+ + O2 | less than 5% conversion | Thermobifida fusca | ? | - |
? | |
2-benzylcyclopentanone + NADPH + H+ + O2 | about 10% conversion | Thermobifida fusca | ? | - |
? | |
2-nonanone + NADPH + H+ + O2 | less than 40% conversion | Thermobifida fusca | ? | - |
? | |
2-octanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
2-phenylcyclohexanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
3-(4-chlorophenyl)cyclobutanone + NADPH + H+ + O2 | about 40% conversion | Thermobifida fusca | ? | - |
? | |
3-benzylcyclobutanone + NADPH + H+ + O2 | about 45% conversion | Thermobifida fusca | ? | - |
? | |
3-octanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
3-phenylcyclobutanone + NADPH + H+ + O2 | about 70% conversion | Thermobifida fusca | ? | - |
? | |
bicyclo[2.2.1]heptan-2-one + NADPH + H+ + O2 | about 5% conversion | Thermobifida fusca | ? | - |
? | |
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
cyclopentanone + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
thioanisole + NADPH + H+ + O2 | - |
Thermobifida fusca | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the wild type enzyme has a melting temperature of 60°C | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
0.3 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
0.9 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.4 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
1.6 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
2.3 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
3 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermobifida fusca |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
2-phenylcyclohexanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
0.37 | - |
2-phenylcyclohexanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
0.48 | - |
2-Octanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
0.8 | - |
Cyclopentanone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca | |
1.6 | - |
Cyclopentanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
9.2 | - |
2-Octanone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
35 | - |
phenylacetone | mutant enzyme P253F/G254A/R258M/L443F, at pH 7.5 and 37°C | Thermobifida fusca | |
37.5 | - |
phenylacetone | wild type enzyme, at pH 7.5 and 37°C | Thermobifida fusca |